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In brief, the organoid cells were seeded and embedded in Matrigel domes and maintained in PDXO medium (Advanced DMEM/F12 with 5% FBS, 10 mM HEPES, 1× Glutamax, 1μg/ml hydrocortisone, 50μg/ml gentamicin, 10μM Y-27632 and 10ng/ml hEGF)

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A breakthrough in neurobiology was the discovery of the proliferative potential of the mature mammalian brain, demonstrated during the research of Joseph Altman et al