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Late assessment: rats with ligated left colic artery and vein that underwent additional colon obstruction for three days (IC + OB rats) In deeply anesthetized rats, in addition to the left colic artery and vein obstruction by two ligations performed as described above, which resulted in a 25-mm blood-deprived colon segment, an additional colon obstruction was made close to the distal ligation using a plastic ring 4 mm in diameter