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The absorbance of the supernatant was measured at 645, 663, and 470 nm to determine chlorophyll a, chlorophyll b, and carotenoids, respectively according to the following equations: 2.4 Quantification of leaf relative water content and electrolyte leakage Leaf relative water content (RWC) was calculated according to Weatherley (1950) using the formula: Where FW = fresh weight, TW = turgid weight, and DW = dry weight