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Although necrosis cannot be completely ruled out, the present study demonstrated that the percent of neuroapoptosis detected by the caspase-3 assay and the TUNEL assay increased as the concentration of ethanol increased from 200 to 500 mM, confirming that lower ethanol ( 4–6 weeks old), which demonstrated that alcohol exposure induced an increase in intracellular levels of cAMP and PKA type II regulatory subunits in the rat brain (20), as well as brain PKA activation in mice (40)