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Although neutralizing antibody titers are indeed associated with protection against infection (primarily because they opsonize pathogens, making it easier for macrophages to recognize them), excessive antibody production can have untoward effects such as vaccine-associated enhanced disease (VAED), potentially mediated by the phenomenon of antibody-dependent enhancement or pathogenic priming

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Additionally, the left hind paw was amputated, and its volume was measured using a water displacement method to assess inflammation