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In roots of seedlings that were exposed to AITC for 72 h roGFP2 fluorescence was almost completely absent (data not shown)
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To experimentally assess the functional consequences of our nrf2aΔ5 mutated allele, we tested its ability to activate the transcription of an ARE-driven firefly luciferase reporter gene following a previously published protocol (Mukaigasa et al, 2012)
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