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Mouse livers were first perfused with Krebs-Ringer buffer containing 100 μM EGTA (MilliporeSigma) to chelate calcium, followed by enzymatic digestion using 60 mg of type I collagenase (Thermo Fisher Scientific) dissolved in 50 mL of DMEM/F12

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Simic, T., Mimic-Oka, J., Ille, K., Dragicevic, D