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Nuclei were pelleted (1300 × g , 15 min, 4 °C) and incubated for 3 h at 50 °C in 5 ml of digestion buffer [800 mM guanidine-HCl, 30 mM Tris-HCl (pH 8.0), 30 mM EDTA, 5% (v/v) Tween-20, 0.5% (v/v) Triton X-100, 1200 U proteinase K]
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