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doi: 10.3389/fimmu.2021.750386 12 MariottiSChiantoreMVTeloniRIacobinoACapocefaloAMicheliniZet al

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In brief, HUVECs were lysed in the Co-IP buffer containing 20 mM Tris-HCl (pH7.5), 100 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, 0.5% protease inhibitor cocktail on ice for 20 min