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For the non-fluorescent RHOA constructs, the above plasmids were re-amplified using RHOA_NOF_F/RHOA_NOF_R and inserted into BamHI/EcoRI digested pUltra-Puro

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Approximately 72 hours after siRNA transfection, cells were either collected for western blot or treated with APR-246 for 72 hours before the Cell Titer Glo cell viability assay

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