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One of the first pathophysiological observations was the increased intestinal permeability in all subtypes of the disease (61–63) seen mostly in the diarrhea-predominant IBS (IBS-D) subgroup (64, 65)

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Add the reconstitution volume slowly along the vial wall rather than directing the stream onto the lyophilised cake, swirl gently until fully dissolved, and don’t vortex as mechanical agitation can cause peptide and protein aggregation