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Overview in chlamydomonas/algae In photosynthetic eukaryotes, nitrate assimilation is performed by two transport and two reduction steps: First, nitrate is transported into the cell, then a cytosolic Nitrate Reductase (NR) catalyzes nitrate reduction to nitrite, which subsequently is transported into the chloroplast, where the enzyme Nitrite Reductase (NiR) catalyzes its reduction to ammonium (Guerrero et al., 1975)
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