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The following equation is used to calculate the concentration of dicobalt edetate (g/100 ml): (T2-T1) x F x 4.06 25 where T 1 = sample titre T 2 = blank titre F = factor for disodium edetate (0.02 mol/l) 5.5.3 Analysis in biological fluids Since dicobalt edetate has not been fully characterized, it is difficult to say whether analysis would be meaningful