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These interactions for 1j might promote tighter binding affinity compared to the endogenous substrate NCA, further indicated by a much lower calculated Vina docking score for 1j (−8.1 vs for NCA) that suggests improved energetic interactions for 1j in the NNMT active site

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A single dose can raise GH and IGF-1 for days, an effect later supported by work on serum protein changes