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Melatonin levels — urinary 6-sulfatoxymelatonin (overnight collection) is the standard research measure Cortisol rhythm — morning and evening salivary cortisol to assess circadian regulation Inflammatory markers — hsCRP, IL-6 as general aging biomarkers Fasting glucose and insulin — epithalamin showed effects on glucose metabolism in aged primates (Khavinson et al., 2005) Subjective Tracking Energy journal — daily 1-10 rating at consistent times Skin quality photos — same lighting, same angle, monthly Recovery from exercise or minor illness — note any changes in bounce-back time Mood and cognitive clarity — brief daily notes Best Practices Documented tracking approaches establish all baselines before the first Epitalon cycle, with biomarkers tested at least 2 weeks before starting

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