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A pretreatment (preventive) approach was used, in which hepatocytes were detected with 200 µM H 2 O 2 for 4 hours after being incubated for 2 hours with fresh garlic, black garlic, atorvastatin, and ascorbic acid
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As a standard method, decapitation rather than anesthesia was preferred for sampling to avoid changes in mRNA levels 24,42
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