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For one-cycle binding measurement of AETX-R302 to ligands diva-p 5 /A*02, diva-p 7 /A*11, p 5 WT /A*02, p 7 WT /A*03 and p 7 WT /A*11, biotinylated ligands were captured on a Series S high-affinity streptavidin (SA) sensor chip, and AETX-R302, diluted in 1xHBS-EP+ to a final concentration of 100 nM, was injected into all 6 flow cells at 25 °C and a flow rate of 30 µL/min