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Mapping the BRD4-ASXL1 interface To assess the importance of the interface residues in the BRD4 ET -ASXL1 EBM complex formation, we substituted R573 and I574 in ASXL1 EBM individually to an aspartate and tested R573D and I574D mutants in 1 H, 15 N HSQC titration experiments and fluorescence assays (Fig