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The column was eluted first using buffer G and subsequently using buffer I (50 mM HEPES-NaOH, pH 7.0, 600 mM NaCl, 5 mM EDTA, 1 mM DTT, 1 mM PMSF, and 2 mM Pefabloc, one tablet of cOmplete EDTA-free protease inhibitor cocktail per 50 mL)