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2.7 Determination of SOD, CAT and glutathione peroxidase (GSH-P X ) concentration After thawing, 200 μL of semen was transferred to a 1.5 mL centrifuge tube and centrifuged at 1600 × g for 10 min at 4 °C

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If all do not distinguish between direct and indirect regulation and only use them as biomarkers of efficacy, it will cause incomparability between the results of studies