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The obtained supernatants (50 μL) were reacted at 37 °C for 30 min in the dark after adding 40 mM Tris-HCl buffer (450 μL) and 10 μM 2′,7′-dichlorofluorescein diacetate (10 μL)

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Anything shorter and you're looking at something like a tattoo needle, and that's not an appropriate tool for injecting a medication