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Mitochondrial function is dispensable for GPX4 inhibition-induced ferroptosis Importantly, although glutaminolysis and mitochondrial TCA cycle/ETC activity contribute to cysteine deprivation-induced ferroptosis, ferroptosis can still be triggered by pharmacological inhibition or genetic elimination of GPX4 when these mitochondrial activities are disrupted

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The bioavailability of glutathione when taken orally is relatively low, as digestive enzymes can break down the compound before it reaches your bloodstream