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Melatonin alleviates neuroinflammation by downregulating pro-inflammatory cytokines and inhibiting microglial over-activation

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This results in substantially higher free peptide bioavailability at the muscle IGF-1R compared to equimolar native IGF-1 in serum-containing research systems — an important consideration for in vitro experimental design where IGFBP concentrations vary by medium formulation and serum lot