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Iron If you have anemia — a condition marked by an abnormally low red blood cell count — your doctor may recommend that you start taking iron supplements, according to MedlinePlus
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The PVDF membrane was subsequently washed (5 min × 5) with tris-buffered saline with tween (TBST) buffer and then incubated with the appropriate secondary antibody (Peroxidase-conjugated goat anti-rabbit IgG (H + L), at a dilution of 1:5000) in a 5% bovine serum albumin solution for 1 h at 25 °C
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