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For histological analysis, the femoral and tibial condyles were fixed in 10% neutral buffered formalin, decalcified with 20% EDTA, embedded in paraffin, and sectioned into 5-μm thick frontal slices
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If you develop signs of high cortisol like central weight gain despite overall weight loss, morning headaches, or mood disturbances, testing cortisol through saliva or 24-hour urine collection may be appropriate
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