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The MethyLight assay quantitatively analyzed the promoter methylation of HIC1 and HOXA9 in cfDNA from tissue samples, cancerous samples, and non-cancerous samples

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It has a homodimeric structure, with each ≈ 470-residue polypeptide chain folded into four domains , from the N-terminal to the C-terminal end: a FAD-binding domain, a NAD + -binding domain, a central domain, and an interface domain