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4.1 Establishing the Reference Interval Data Source: Image 3 (“NADs: NORMAL RANGE IN BLOOD”) NAD+ Range: 20–36 µM NADH Range: 0.5–1.8 µM Sample Size: N=300 Statistical Insight: The distribution histograms in Image 3 show a Gaussian (Normal) distribution for both metabolites

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neurotypical children, and the association with autism severity