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Free thiols are then alkylated with a light ( 12 C) ICAT reagent, followed by reduction of with TCEP, which serves to reduce reversible modifications (Chart 1)

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As described by Masarone et al., ED is a result of excessive production of nitric oxide (NO), which in turn contributes to and promotes the structural and functional changes of liver circulation, impairs regeneration after liver injury, and thereby leads to the progression of liver disease [50]

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