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Technical support was provided by FIOCRUZ Network of Technological Platforms: Real Time PCR and Digital PCR (P04-006), NGS Sequencing (P01-007), and Bioinformatics (P08-002)

Group 1

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followed by a three prime untranslated region (bases 3880–4174) combined from AES and mtRNR1 selected for increased protein expression and mRNA stability [129] and a poly(A) tail comprising 30 adenosine residues, a 10-nucleotide linker sequence, and 70 other adenosine residues (bases 4175–4284)